VersaTaq Direct PCR Polymerase amplifies DNA from as high as 40% human whole blood. Hemoglobin, a known inhibitor of polymerase activity in PCR reactions, poses a serious challenge for clinical and forensic PCR applications.

VersaTaq Direct PCR Polymerase can be used in end-point PCR applications directly with many potent PCR inhibitors such as those found in: Moreover, this enzyme has 5’->3’ exonuclease activity, making it suitable for hydrolysis probe-based detection chemistries. VersaTaq Direct PCR Polymerase works with a wide range of samples that would pose an issue for a regular Taq polymerase. With the VersaTaq Direct PCR Polymerase protocol, you start directly with PCR setup in a few simple steps.įigure 1. This mutant Taq jump starts your setup directly to the PCR step, eliminating costly extraction steps. Unlike standard Taq DNA polymerases, VersaTaq Direct PCR Polymerase is resistant to many potent PCR inhibitors. These steps can be time-consuming, may result in the loss of the starting material, and sometimes failed amplification. Thus, a standard Taq DNA polymerase requires procedures to extract and purify DNA prior to PCR. Taq DNA polymerase activity is blocked by PCR inhibitors, like hemoglobin and humic acid, which are found in blood and soil. Save money and time by avoiding messy cleanup steps Can be used with a variety of sample sources.Shorter experiment times leading to faster data output.Eliminates costly extraction and purification steps.Here are key features of this versatile enzyme: USB ® products from Affymetrix ®, the leader in enzymatic PCR cleanup, has introduced a new mutant Taq polymerase, VersaTaq Direct PCR Polymerase, which enables direct amplification from samples containing potent PCR inhibitors. Are you spending too much time and money extracting and purifying DNA samples for PCR analysis? Now you can avoid the messy cleanup steps and go direct to DNA amplification from a wide range of difficult sample types.

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